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Table 1 Synthesis of UDP-GlcA from starch through a cascade using whole cells expressing hyperthermophilic enzymes

From: Cascade synthesis of uridine-5′-diphosphate glucuronic acid by coupling multiple whole cells expressing hyperthermophilic enzymes

Entry Pro . Sub. Sub. conc. (mM) NAD+ (mM) UTP (mM) Whole-cells (DCW, g/L) Time (h) Conv. of sub. (%) Conc. of product (mM)
1a G1P Starch 5% (w/v) TmαGP (9.2) 6 22 40
2b UDP-Glc G1P 10 10 TmαGP (2.3) StUSP (13.6) 3 46 4.6
3c UDP-GlcA UDPG 2.3 TmαGP (1.1) StUSP (6.8) PiUDH (14.6) 0.5 52 1.2
4c UDP-GlcA UDPG 2.3 0.1 TmαGP (1.1) StUSP (6.8) PiUDH (14.6) 0.5 100 2.3
5c UDP-GlcA UDPG 2.3 TmαGP (1.1) StUSP (6.8) PiUDH (14.6) TkNOX (8.8) 0.5 100 2.3
6c UDP-GlcA UDPG 2.3 TmαGP (1.1) StUSP (6.8) PiUDH-TkNOX (17.8) 0.5 100 2.3
  1. Data are the average of triplicate measurements
  2. aEntry 1 was incubated in a reaction mixture (pH 7.0) at 70 °C for 6 h
  3. bEntry 2 was incubated in a reaction mixture (pH 9.0) at 80 °C for 3 h
  4. cEntries 3, 4, 5 and 6 were incubated in a reaction mixture (pH 10.0) at 65 °C for 0.5 h