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Table 1 Synthesis of UDP-GlcA from starch through a cascade using whole cells expressing hyperthermophilic enzymes

From: Cascade synthesis of uridine-5′-diphosphate glucuronic acid by coupling multiple whole cells expressing hyperthermophilic enzymes

Entry

Pro .

Sub.

Sub. conc. (mM)

NAD+ (mM)

UTP (mM)

Whole-cells (DCW, g/L)

Time (h)

Conv. of sub. (%)

Conc. of product (mM)

1a

G1P

Starch

5% (w/v)

TmαGP (9.2)

6

22

40

2b

UDP-Glc

G1P

10

10

TmαGP (2.3)

StUSP (13.6)

3

46

4.6

3c

UDP-GlcA

UDPG

2.3

TmαGP (1.1)

StUSP (6.8)

PiUDH (14.6)

0.5

52

1.2

4c

UDP-GlcA

UDPG

2.3

0.1

TmαGP (1.1)

StUSP (6.8)

PiUDH (14.6)

0.5

100

2.3

5c

UDP-GlcA

UDPG

2.3

TmαGP (1.1)

StUSP (6.8)

PiUDH (14.6)

TkNOX (8.8)

0.5

100

2.3

6c

UDP-GlcA

UDPG

2.3

TmαGP (1.1)

StUSP (6.8)

PiUDH-TkNOX (17.8)

0.5

100

2.3

  1. Data are the average of triplicate measurements
  2. aEntry 1 was incubated in a reaction mixture (pH 7.0) at 70 °C for 6 h
  3. bEntry 2 was incubated in a reaction mixture (pH 9.0) at 80 °C for 3 h
  4. cEntries 3, 4, 5 and 6 were incubated in a reaction mixture (pH 10.0) at 65 °C for 0.5 h