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Fig. 6 | Microbial Cell Factories

Fig. 6

From: A toolset of constitutive promoters for metabolic engineering of Rhodosporidium toruloides

Fig. 6

Correlation of fluorescence from two reporters expressed from R. toruloides promoters. To get a sense of promoter robustness, each promoter was cloned in front of two reporter genes, EGFP and mRuby2 (denoted as orientation 1 and 2 in Fig. 1). Average fluorescence output for both reporters at 48 h is charted for each promoter in four different media: correlation between EGFP expression from strains containing constructs in orientation 1 and mRuby2 expression from strains containing constructs in orientation 2. Solid line is the linear regression and dashed lines are the 95% confidence interval. YPD (a); SD supplemented with 1% (w/v) each of glucose and xylose (b); SD supplemented with 1% (w/v) glucose (c); and SD supplemented with 1% (w/v) xylose (d). Pearson correlation for promoter expression in SD with 1% glucose and 1% xylose resulted in a R2 of 0.6159 and for SD 1% glucose resulted in a R2 of 0.7223, both with a p value of < 0.0001. Correlation of promoter expression in SD 1% xylose resulted in a R2 of 0.471 and in YPD resulted in a R2 of 0.5041, both with a p value of 0.0005

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