Table 1 The analysis of enzyme reaction by HPLC and LC–ESI–MS
From: Construction of Escherichia coli cell factories for crocin biosynthesis
Enzyme reaction | Product peaks of analysis (min) | m/z (By LC–MS) | Reactants |
|---|---|---|---|
Crocetin + YdhE | 26.40 28.80 | 491.22, 653.27 | Crocin-5, Crocin-4/Crocin-3 |
Crocetin + YjiC | 27.70 | 653.27 | Crocin-4/Crocin-3 |
Crocetin + YojK | 25.70, 28.00 | 491.22, 653.27, 815.33, 977.38 | Crocin-5, Crocin-4, Crocin-3, Crocin-2, Crocin-1 |