Table 4 Source of key mutations validated for causality in isolated strains from the current study or constructed previously from Lacroix et al. [22]

K-12 MG1655

N/A

N/A

pyrE-rph

Δ82 bp deletion

LaCroix et al. [22]

rpoB

E672K (GAA→AAA)

LaCroix et al. [22]

rpoC

R1075C (CGT→TGT)

MGcscBKAp A1 F138 I1

MGcscBKAp A2 F140 I1

R978C (CGT→TGT)

MGcscBKAp A3 F139 I1

T1045P (ACC→CCC)

MGcscBKA A3 F140 I1 with additional mutations of (tdcG, baeS)

pyrE-rph + rpoB

Δ82 bp + E672K (GAA→AAA)

LaCroix et al. [22]

pyrE-rph + rpoB

Δ82 bp + S621F (TCC→TTC)

MGcscBKA A1 F136 I1