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Table 3 Key mutations found after evolution of E. coli on sucrose

From: Generation of an E. coli platform strain for improved sucrose utilization using adaptive laboratory evolution

Strain

Gene or genetic region

Mutation

Mutation type

Function

Number of independent occurrences

Sample ID (frequency of mutation in population samples)

K-12 starting strains MGcscBKA and MGcscBKAp

pyrE/rph

Δ82 bp intragenic

DEL

Orotate phosphoribosyltransferase/ribonuclease PH

2

MGcscBKA A1 F136 I0 (43%), MGcscBKA A1 F136 I1, MGcscBKA A3 F140 I0 (25%)

T→G intergenic (− 47/+ 19)

SNP

1

MGcscBKAp A3 F139 I0 (71%)

rpoB

S621F (TCC→TTC)

SNP

RNA polymerase subunit ÎČ

1

MGcscBKA A1 F136 I0 (48%), MGcscBKA A1 F136 I1

Q618L (CAG→CTG)

SNP

1

MGcscBKA A2 F134 I0 (4%)

rpoC

T1045P (ACC→CCC)

SNP

RNA polymerase subunit ÎČ’

2

MGcscBKA A3 F140 I0 R1 (20%), MGcscBKA A3 F140 I1 R1, MGcscBKAp A3 F139 I0 (83%)

R1075C (CGT→TGT)

SNP

2

MGcscBKAp A1 F138 I0 (93%), MGcscBKAp A1 F138 I1, MGcscBKAp A2 F140 I0 (100%), MGcscBKAp A2 F140 I1

L770R (CTC→CGC)

SNP

1

MGcscBKA A3 F140 I0 (76%)

R978C (CGT→TGT)

SNP

1

MGcscBKAp A3 F139 I0 (9.7%), MGcscBKAp A3 F139 I1

Q665K (CAG→AAG)

SNP

1

MGcscBKA A2 F134 I0 (6%)

R1174P (CGT→CCT)

SNP

1

MGcscBKA A2 F134 I0 (18%)

E. coli W

cscR

Δ1403 bp [cscR, dsdX, dsdA]

DEL

Sucrose operon repressor (Csc operon regulatory protein), permease DsdX, d-serine dehydratase (EC:4.3.1.18)

1

E. coli W A1 F155 I1

Δ84 bp

DEL

Sucrose operon repressor (Csc operon regulatory protein)

1

E. coli W A3 F156 I1

Δ10 bp

DEL

 

1

E. coli W A1 F155 I0 (74%)

Δ21 bp

DEL

Sucrose operon repressor (Csc operon regulatory protein)

1

E. coli W A3 F156 I0 (46%)

mrdB

S31R (AGC→AGG)

SNP

Rod shape-determining protein RodA

1

E. coli W A1 F155 I0 (77%)

S37R (AGC→AGA)

SNP

1

E. coli W A2 F158 I0 (81)

E270Q (GAA→CAA)

SNP

1

E. coli W A2 F158 I1

mrdA

W434C (TGG→TGC)

SNP

Penicillin-binding protein 2

1

E. coli W A1 F155 I1

  1. The sample ID has a unique identifier—(A) refers to the independent replicate, (F) refers to the flask number, and (I) to the type of the isolate: population (0) or clone (1)
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Microbial Cell Factories

ISSN: 1475-2859

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