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Table 3 Key mutations found after evolution of E. coli on sucrose

From: Generation of an E. coli platform strain for improved sucrose utilization using adaptive laboratory evolution

Strain Gene or genetic region Mutation Mutation type Function Number of independent occurrences Sample ID (frequency of mutation in population samples)
K-12 starting strains MGcscBKA and MGcscBKAp pyrE/rph Δ82 bp intragenic DEL Orotate phosphoribosyltransferase/ribonuclease PH 2 MGcscBKA A1 F136 I0 (43%), MGcscBKA A1 F136 I1, MGcscBKA A3 F140 I0 (25%)
T→G intergenic (− 47/+ 19) SNP 1 MGcscBKAp A3 F139 I0 (71%)
rpoB S621F (TCC→TTC) SNP RNA polymerase subunit β 1 MGcscBKA A1 F136 I0 (48%), MGcscBKA A1 F136 I1
Q618L (CAG→CTG) SNP 1 MGcscBKA A2 F134 I0 (4%)
rpoC T1045P (ACC→CCC) SNP RNA polymerase subunit β’ 2 MGcscBKA A3 F140 I0 R1 (20%), MGcscBKA A3 F140 I1 R1, MGcscBKAp A3 F139 I0 (83%)
R1075C (CGT→TGT) SNP 2 MGcscBKAp A1 F138 I0 (93%), MGcscBKAp A1 F138 I1, MGcscBKAp A2 F140 I0 (100%), MGcscBKAp A2 F140 I1
L770R (CTC→CGC) SNP 1 MGcscBKA A3 F140 I0 (76%)
R978C (CGT→TGT) SNP 1 MGcscBKAp A3 F139 I0 (9.7%), MGcscBKAp A3 F139 I1
Q665K (CAG→AAG) SNP 1 MGcscBKA A2 F134 I0 (6%)
R1174P (CGT→CCT) SNP 1 MGcscBKA A2 F134 I0 (18%)
E. coli W cscR Δ1403 bp [cscR, dsdX, dsdA] DEL Sucrose operon repressor (Csc operon regulatory protein), permease DsdX, d-serine dehydratase (EC:4.3.1.18) 1 E. coli W A1 F155 I1
Δ84 bp DEL Sucrose operon repressor (Csc operon regulatory protein) 1 E. coli W A3 F156 I1
Δ10 bp DEL   1 E. coli W A1 F155 I0 (74%)
Δ21 bp DEL Sucrose operon repressor (Csc operon regulatory protein) 1 E. coli W A3 F156 I0 (46%)
mrdB S31R (AGC→AGG) SNP Rod shape-determining protein RodA 1 E. coli W A1 F155 I0 (77%)
S37R (AGC→AGA) SNP 1 E. coli W A2 F158 I0 (81)
E270Q (GAA→CAA) SNP 1 E. coli W A2 F158 I1
mrdA W434C (TGG→TGC) SNP Penicillin-binding protein 2 1 E. coli W A1 F155 I1
  1. The sample ID has a unique identifier—(A) refers to the independent replicate, (F) refers to the flask number, and (I) to the type of the isolate: population (0) or clone (1)