Generation of an E. coli platform strain for improved sucrose utilization using adaptive laboratory evolution

Table 1 Properties of the ALE experiments end point populations

ALE experiment	Strain (population)	Total CCD x10¹²	Ratio of fitness increase to start strain
K-12 MGcscBKA	1	8.65	1.72
	2	8.75	1.46
	3	8.94	1.61
K-12 MGcscBKAp	1	9.02	1.48
	2	9.02	1.47
	3	8.85	1.48
E. coli W	1	9.71	1.29
	2	9.78	1.31
	3	9.87	1.22

Ratios of fitness increase were determined from growth rates that were calculated based on the last three flasks during exponential growth. CCD, cumulative cell divisions

ISSN: 1475-2859