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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Generation of an E. coli platform strain for improved sucrose utilization using adaptive laboratory evolution

Fig. 5

Causal mutation analysis for fitness improvement on sucrose and glucose for K-12 strains. Shown is a bar graph representing growth rate of identified causal mutations from the current study (ALE on sucrose) and LaCroix et al. [22] (ALE on glucose). The error bars are calculated from two biological replicates (n = 2). All rpoC mutations originated from this study, as well as the rpoB S621F with the pyrE-rph deletion mutation (i.e., they were not reverse engineered). The remaining mutations were from glucose ALE control experiment (GLUALE) with chromosomally integrated csc gene cassette as described in the methods

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