Fig. 5From: Generation of an E. coli platform strain for improved sucrose utilization using adaptive laboratory evolutionCausal mutation analysis for fitness improvement on sucrose and glucose for K-12 strains. Shown is a bar graph representing growth rate of identified causal mutations from the current study (ALE on sucrose) and LaCroix et al. [22] (ALE on glucose). The error bars are calculated from two biological replicates (nâ=â2). All rpoC mutations originated from this study, as well as the rpoB S621F with the pyrE-rph deletion mutation (i.e., they were not reverse engineered). The remaining mutations were from glucose ALE control experiment (GLUALE) with chromosomally integrated csc gene cassette as described in the methodsBack to article page