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Table 1 Reactions of a metabolic network

From: A kinetic metabolic study of lipid production in Chlorella protothecoides under heterotrophic condition

No.

Enzyme

Description

Reaction

1

HK

Hexokinase

EGLC  G6P

2

GPI

Glucose 6 phosphate isomerase

G6P   F6P

3

PFK

6 phosphofructokinase

F6P   2 GD

4

FBPase

Fructose biphosphate aldolase

2 GD   F6P

5

PGK

Phosphoglycerate kinase

GD   PEP

6

PK

Pyruvate kinase

PEP   PYR

7

PDH

Pyruvate dehydrogenase

PYR   AcCOA + CO2

8

FASN

Fatty acid synthase

12 AcCOA   Lipid

9

Lipase

Lipase

Lipid   12 AcCOA

10

GPAT

Glycerol-3-phosphate acyltransferases

GlyP = Lipid

11

TPI

Triosephosphate isomerase

GD   GlyP

12

G6PDH

Glucose 6 phosphate 1 dehydrogenase

G6P   RX + CO2

13

TK

Transketolase

3 RX   2 F6P + GD

14

PPRiBP

Phosphoribosyl-diphosphate synthetase

RX = ADP

15

CK

Creatine kinase

ADP   ATP

16

AK

Adenylate kinase

ATP   ADP

17

PGM

Phosphoglucomutase

G6P = G1P

18

ADPG

Adenosine diphosphate glucose-starch glucosyltransferase

25 G1P   Starch

19

AP

Amylase

Starch   25 G1P

20

GHMT

Glycine hydroxymethyltransferase

GLY   PYR

21

CS

Citrate synthase

AcCOA   CIT

22

MLD

Malate dehydrogenase

MAL   CIT

23

ISOD

Isocitrate dehydrogenase

CIT   AKG + CO2

24

AKGDH

Oxoglutarate dehydrogenase

AKG   SCOA + CO2

25

GLDH

Glutamate dehydrogenase

AKG = AA

26

SCOAS

Succinyl CoA ligase

SCOA   SUCC

27

SDH

Succinate dehydrogenase

SUCC   FUM

28

FH

Fumarate hydratase

FUM   MAL

29

ME

Malic enzyme

MAL   PEP + CO2

30

Growth

Biomass synthesis

G6P + RX + PYR + Lipid + AcCOA  X

  1. ‘ ’, represents unidirectional reactions; =’, represents reversible reactions