Fig. 7

Shown are the specific product yields of pSET152-based acbC-overexpression mutants grown in a shake flask cultivation in maltose minimal medium in a bar diagram. Each specific product yield is calculated from the final acarbose concentration normalized to the final cell dry weight and cultivation time. Error was estimated by Gaussian error propagation. Final cell dry weights of each mutant are shown by a dot plot. Detailed growth curves and acarbose concentrations over time are shown in the Additional file 1: Data S5