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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Evaluation of vector systems and promoters for overexpression of the acarbose biosynthesis gene acbC in Actinoplanes sp. SE50/110

Fig. 5

Relative transcript amounts of the gusA gene under control of different homologous and heterologous promoters in Actinoplanes sp. SE50/110. The RNA was isolated from the growth phase of a shake flask cultivation in maltose minimal medium and analyzed by RT-qPCR. The calculated averages and standard deviations of a minimum of three biological replicates are shown. The transcription amounts of gusA gene are analyzed in relation to the amounts of the Actinoplanes sp. SE50/110 mutant carrying the promoterless pGUS-vector (relative transcript amount of promoterless pGUS set to 1). For actP no RNA could be isolated due to a severe growth deficiency in maltose minimal medium. (p-values of a two-sided t-test: P2475: 0.0001133, Pefp: 4.871e−05, PcdaR: 0.002509, PrpsL: 9.928e−06, PrpsJ: 1.167e−08, Pcgt: 5.911e−08, PtipA: 7.158e−06, Papm: 4.596e−05, PermE*: 0.0009364, PkatE: 0.0001373, PmoeE5: 0.0002518, PgapDH: 4.207e−06)

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Microbial Cell Factories

ISSN: 1475-2859

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