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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Evaluation of vector systems and promoters for overexpression of the acarbose biosynthesis gene acbC in Actinoplanes sp. SE50/110

Fig. 4

Relative transcript amounts of single genes in different pSET152-mutants of Actinoplanes sp. SE50/110. The genes acbA, acbB, acbWXY, zwf and cgt were transcribed under control of their native promoter. In case of acbC and acbS the promoter upstream of acbV was used, which is the first gene of the respective operon. The RNA was isolated from triplicates of the growth phase of a shake flask cultivation in maltose minimal medium and analyzed by RT-qPCR. The relative transcript amounts were analyzed in relation to the amounts of the empty vector control Actinoplanes sp. SE50/110 [pSET152] (relative transcript amount set to 1)

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Microbial Cell Factories

ISSN: 1475-2859

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