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Fig. 10 | Microbial Cell Factories

Fig. 10

From: Evaluation of vector systems and promoters for overexpression of the acarbose biosynthesis gene acbC in Actinoplanes sp. SE50/110

Fig. 10

In the overexpression mutant of acbC (gapDH- or rpsL-promoter), a peak at 12.6 [min] arises (orange and blue), with the specific mass m/z = 255.03 [M−H+]. The corresponding peak is decisively higher compared to the empty vector control and a pSET152-construct with acbC under control of its native promoter (grey and black). According to MS/MS, this compound is phosphorylated (data not shown). In Actinoplanes sp. SE50/110 [pKC1139::PermE*::acbL] (pink), in which the acb gene cluster was disrupted by unintended vector integration, this compound does not occur

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Microbial Cell Factories

ISSN: 1475-2859

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