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Table 1 Strains and plasmids used in this study

From: Promoter engineering enables overproduction of foreign proteins from a single copy expression cassette in Bacillus subtilis

Strain or plasmid

Characteristicsa

Source or referenceb

Plasmids

 pAX01

PxylA, Apr, Emr, lacA

[15]

 pYBGB

pAX01 containing Pylb-bgaB cassette

This work

 pLJ-2

CmR; E. coli-Bacillus shuttle vector

[19]

 pUS20

Spcr, unstable in B. subtilis, E. coli-Bacillus shuttle vector

[71]

Strains

 E. coli Top10

F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu)7697 galU galK endA1 nupG

TransGene Biotech (Beijing, China)

B. subtilis

 168

trpC2

BGSC 1A1

 WB800

(168) ΔnprE, Δ aprA, Δepr, Δ bpr, Δmpr, ΔnprB, Δvpr, ΔwprA

[72]

 WBEmBgaB

Strain WB800 derivate, lacA::Pylb-bgaB, Emr

This work

 WBBgaB

Strain WB800 derivate, lacA::Pylb-bgaB

This work

 BS43

Strain WB800 derivate, lacA::P43-bgaB

This work

 BSxylA

Strain WB800 derivate, lacA::PxylA-bgaB

This work

 BSsrfA

Strain WB800 derivate, lacA::PsrfA-bgaB

This work

 35BgaB

Strain WB800 derivate, lacA::P35-bgaB

This work

 10BgaB

Strain WB800 derivate, lacA::P10-bgaB

This work

 3510BgaB

Strain WB800 derivate, lacA::P3510-bgaB

This work

 351016BgaB

Strain WB800 derivate, lacA::P351016-bgaB

This work

 351022BgaB

Strain WB800 derivate, lacA::P351022-bgaB

This work

 OBgaB

Strain WB800 derivate, lacA::OP3510-bgaB

This work

 JBgaB

Strain WB800 derivate, lacA::JP3510-bgaB

This work

 DBgaB

Strain WB800 derivate, lacA::DP3510-bgaB

This work

 BBgaB

Strain WB800 derivate, lacA::BP3510-bgaB

This work

 WBSBgaB

Strain WB800 derivate, lacA::NBP3510-bgaB

This work

 WBGFP

Strain WB800 derivate, lacA:: Pylb -sfGFP

This work

 WBSGFP

Strain WB800 derivate, lacA::NBP3510-bgaB

This work

 WBSMPH

Strain WB800 derivate, lacA::NBP3510-mpd

This work

 WBSChd

Strain WB800 derivate, lacA::NBP3510-chd

This work

  1. aApR: ampicillin resistance; CmR: chloramphenicol resistance; EmR: erythromycin resistance; Spcr: spectinomycin resistance
  2. bBGSC: Bacillus Genetic Stock Center