Fig. 1From: Promoter engineering enables overproduction of foreign proteins from a single copy expression cassette in Bacillus subtilisa Construction of the integrative plasmid pYBGB. b Comparison of the maximum yield of BgaB under the control of different promoters. All cultures were grown in triplicate, and each experiment was performed at least twice. Error bars indicate standard deviations. c The expression level and pattern of BgaB measured in strain WBBgaB. During 24 h of cultivation, cells were sampled periodically and analyzed by examining the biomass and BgaB activityBack to article page