Strains/plasmids | Characteristics | Sources/references |
---|---|---|
Strains | ||
L. brevis ATCC 367 | Wild-type strain | ATCC |
L. brevis D17 | Wild-type strain | This study |
L. brevis ATCC 367ΔgadR | Derivative of L. brevis ATCC 367 with gadR deletion | This study |
L. brevis D17ΔgadR | Derivative of L. brevis D17 with gadR deletion | This study |
E. coli Top10 | Recipient for cloning experiments | Invitrogen |
Plasmids | ||
pGID023 | Integration vector, Emr (erythromycin resistance gene) | [38] |
pGID023-AB | pGID023 carrying 2 kb DNA fragments derived from upstream and downstream region of the gadR gene | This study |