Fig. 8From: Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastorisFed-batch cultivation of HyHEL-Fab#34 overexpressing SBH1 and deleted for ypt7 or vam6pep4 and combinations thereof. The product titers (a, c) of all the strains were determined by ELISA and measured at several time points during the fed-batch process. The dry cell weight (b, d) was assessed at similar time points of the cultivationBack to article page