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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris

Fig. 7

Screening of HyHEL-Fab#34 overexpressing KAR2, SBH1 or RHO4, and Δvam6Δpep4 or Δypt7 overexpressing SBH1. The relative fold changes of extracellular HyHEL-Fab titers (a) and HyHEL-Fab yields (b) were determined by ELISA and calculated from one screening containing 12 clones of each strain including the empty vector control strain Fab#34. The relative fold changes of secreted HyHEL-Fab titers (c) and HyHEL-Fab yields (d) in the HOPS mutants were determined by ELISA and calculated from one screening containing the 4 best performing clones of Δvam6Δpep4 + SBH1OE, Δypt7 + SBH1OE, SBH1OE and Fab#34 strains. Error bars indicate the standard error of the mean (SEM). Statistical significance measured by the Student’s t-test is indicated with an asterisk (p < 0.05*, p < 0.01**, p < 0.001***)

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