Fig. 6

Intracellular accumulation of the recombinant protein in wt, Δypt7 and Δvps41 strains. Intracellular levels of HyHEL-Fab (a) and CES (b) were detected by Western blot of cell lysates. Equal amounts of total intracellular protein (determined by BCA assay after cell lysis) were loaded on the gel. c Localization of the recombinant protein in P. pastoris overexpressing CES-oxGFP in wt, Δypt7 and Δvps41 strains. After cultivation cells were put directly on a slide and viewed in an epifluorescence microscope. Bar, 5 µm