Fig. 3

Screening of ∆ypt7, ∆vam6 and ∆vps41 strains overexpressing HyHEL-Fab or CES along with their respective control strains Fab#34 and CES#18. Detection of extracellular HyHEL-Fab (a) and CES (b) by ELISA and Western blot. For the ELISA, the relative fold changes of the yield were calculated from 3 to 5 biological and technical replicates each. Error bars represent the SEM. Statistical significance was measured by the Student’s t test and indicated with an asterisk (p < 0.05*, p < 0.01**). Prior to loading the samples on the Western blot, the protein amounts were normalized to the wet cell weight of each strain