Fig. 1

Effect of ∆atg8 or ∆atg11 on model protein secretion in Fab#34 and CES#18. Detection of secreted HyHEL-Fab (a) and CES (b) by ELISA and Western blot. Prior to loading, the protein amounts were normalized to the wet cell weight of each strain. For ELISA, the relative fold changes of extracellular Fab yields were calculated from one screening with 2 ∆atg8, 2 ∆atg11 clones and 2 Fab#34 biological replicates whereas the relative fold changes of extracellular CES yields were calculated from two screenings with 4 ∆atg8, 4 ∆atg11 clones and 2 CES#18 biological replicates in each screening. Error bars indicate the standard error of the mean (SEM)