Skip to main content

Advertisement

Fig. 1 | Microbial Cell Factories

Fig. 1

From: Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris

Fig. 1

Effect of ∆atg8 oratg11 on model protein secretion in Fab#34 and CES#18. Detection of secreted HyHEL-Fab (a) and CES (b) by ELISA and Western blot. Prior to loading, the protein amounts were normalized to the wet cell weight of each strain. For ELISA, the relative fold changes of extracellular Fab yields were calculated from one screening with 2 ∆atg8, 2 ∆atg11 clones and 2 Fab#34 biological replicates whereas the relative fold changes of extracellular CES yields were calculated from two screenings with 4 ∆atg8, 4 ∆atg11 clones and 2 CES#18 biological replicates in each screening. Error bars indicate the standard error of the mean (SEM)

Back to article page