Fig. 3From: Production of trehalose with trehalose synthase expressed and displayed on the surface of Bacillus subtilis sporesLocalization of CotG-TreS, CotC-TreS, and CotC-TreS–CotG-TreS fusion proteins as assessed by immunofluorescence microscopy. Spores were incubated with the mouse anti-His tag antibody (1:60, primary antibody), followed by probing with the FITC-conjugated goat anti-rabbit IgG antibody (1:30, secondary antibody). The same exposure time was used for all immunofluorescence images. Photos from left to right correspond to white, fluorescent, and white-light superpositionBack to article page