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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Production of trehalose with trehalose synthase expressed and displayed on the surface of Bacillus subtilis spores

Fig. 3

Localization of CotG-TreS, CotC-TreS, and CotC-TreS–CotG-TreS fusion proteins as assessed by immunofluorescence microscopy. Spores were incubated with the mouse anti-His tag antibody (1:60, primary antibody), followed by probing with the FITC-conjugated goat anti-rabbit IgG antibody (1:30, secondary antibody). The same exposure time was used for all immunofluorescence images. Photos from left to right correspond to white, fluorescent, and white-light superposition

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