Fig. 4From: Intranasal administration with recombinant Bacillus subtilis induces strong mucosal immune responses against pseudorabiesThe proliferation of lymphocytes in spleen. Splenocytes were prepared at 35 day and cultured with PBS, Con A, B. subtilis-PRV, B. subtilis-gCa and B. subtilis-gDa for 72 h at 37 °C. Then, the proliferative responses detected by CCK8 method and shown as a proliferation ratio. Data were expressed as the mean ± SD. **P < 0.01Back to article page