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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Development of a novel strategy for robust synthetic bacterial promoters based on a stepwise evolution targeting the spacer region of the core promoter in Bacillus subtilis

Fig. 2

The mutagenesis and characterization of pPML. a Comparison of GFP expression driven by promoter P43 and PsrfA. b SDS-PAGE analysis of GFP expression driven by P43 and PsrfA. c Verification of the fluorescence intensity of the four representative mutants at the 24-h cultivation in the scale of the shake flask. The experiments were repeated independently in triplicate. The data were shown by mean ± S.D. d SDS-PAGE analysis of the GFP expression levels in the four selected mutants. The whole-cell proteins of each sample were separated by 12% SDS-PAGE and were then stained with Coomassie Brilliant Blue R-250. CK denoted the strain without any plasmid

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Microbial Cell Factories

ISSN: 1475-2859

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