Skip to main content
Fig. 1 | Microbial Cell Factories

Fig. 1

From: Development of a novel strategy for robust synthetic bacterial promoters based on a stepwise evolution targeting the spacer region of the core promoter in Bacillus subtilis

Fig. 1

Schematic diagram of the SETarSCoP strategy. a Conservation analysis of spacer sequences between − 35 box and − 10 box of strong promoters and weak promoters. Conserved sequence was generated by WebLogo (http://weblogo.berkeley.edu/logo.cgi). The target regions for mutagenesis in the first and the second round of evolution were encircled by blue and orange dashed rectangle, respectively. b The scheme of SETarSCoP strategy performed by two rounds of evolution. The 3-bp sequence upstream of the − 10 box was randomly mutated at the first-round evolution. After screening, the positively evolved (being stronger than the parental promoter) mutants were chosen and validated. Subsequently, these evolved mutants were used as templates, 4-bp sequence immediately upstream of the region of the first-round mutation was used to perform random mutagenesis in the second-round evolution

Back to article page