Skip to main content

Advertisement

Table 3 Enzymatic properties of wild-type enzymes and fusion proteins

From: An efficient expression tag library based on self-assembling amphipathic peptides

Enzyme Value ± SD
Km (g L−1#, mM*, μmol L−1″) kcat (min−1#, S−1*, S−1″) Specific activity (U mg−1) t1/2 (min)
PGL 0.27 ± 0.011 12.69 ± 0.4 279.14 ± 3.5 5.3 ± 0.32
S1nv10-PGL 0.47 ± 0.07 65.6 ± 6.8 499.6 ± 8.4 16.4 ± 1.3
LOX 0.057 ± 0.004 24.7 ± 2.97 30.2 ± 1.22 9.7 ± 0.43
S1nv11-LOX 0.052 ± 0.006 81.2 ± 3.6 38.23 ± 1.87 46.8 ± 1.5
ASN 14.2 ± 0.31 49.2 ± 1.9 17.34 ± 0.16 12.3 ± 0.42
S1nv17-ASN 21.4 ± 0.34 68.6 ± 2.5 21.34 ± 0.6 24.4 ± 0.65
  1. Each result was the average value of three parallel experiments. SD is the standard deviation of three experiments
  2. #, * and " indicated the units for PGL, LOX, and ASN, respectively