Table 3 Enzymatic properties of wild-type enzymes and fusion proteins
From: An efficient expression tag library based on self-assembling amphipathic peptides
Enzyme | Value ± SD | |||
|---|---|---|---|---|
Km (g L−1#, mM*, μmol L−1″) | kcat (min−1#, S−1*, S−1″) | Specific activity (U mg−1) | t1/2 (min) | |
PGL | 0.27 ± 0.011 | 12.69 ± 0.4 | 279.14 ± 3.5 | 5.3 ± 0.32 |
S1nv10-PGL | 0.47 ± 0.07 | 65.6 ± 6.8 | 499.6 ± 8.4 | 16.4 ± 1.3 |
LOX | 0.057 ± 0.004 | 24.7 ± 2.97 | 30.2 ± 1.22 | 9.7 ± 0.43 |
S1nv11-LOX | 0.052 ± 0.006 | 81.2 ± 3.6 | 38.23 ± 1.87 | 46.8 ± 1.5 |
ASN | 14.2 ± 0.31 | 49.2 ± 1.9 | 17.34 ± 0.16 | 12.3 ± 0.42 |
S1nv17-ASN | 21.4 ± 0.34 | 68.6 ± 2.5 | 21.34 ± 0.6 | 24.4 ± 0.65 |