Fig. 5

Heat map of the biosensor Fluorescence Intensity (FI) normalized to the autofluorescence of each background strain (TMB37X1; no biosensor). a HXT1p, b SUC2p and c TPS1p. Each biosensor was normalized to the corresponding deletion in the TMB37X1 strains to account for changes in autofluorescence due to changes in morphology caused by the deletions, e.g. TMB3772 (HXT1p) was normalized to TMB3771 (no biosensor). The conditions were: repression according to [39], xylose 50 g/L (X50), glucose 5 g/L (G5) and YNB without carbon source (YNB only). SUC2p (b) displayed subpopulations during cultivation on xylose 50 g/L in some of the strains, and therefore one row for each subpopulation is displayed for X50; note that TMB3755 and TMB3775 only had one population, which is indicated by the same colour in both subpopulation rows. This figure should be interpreted along Fig. 4, since the heat map does not take the strength and potential constitutive expression of the FI signal into account, only the fold change. *The TPS1p-GFP biosensor has previously been shown to be difficult to repress in the background strain [38, 39], and does therefore have a fold change > 1 in the 0 h repression condition in TMB3757