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Table 3 The production, volumetric productivity and yield of the parent strains and the recombinant strains with racE, murC, yrpC, H1, H2, and H3

From: Enhancing surfactin production by using systematic CRISPRi repression to screen amino acid biosynthesis genes in Bacillus subtilis

Strains

Target genes

Titre

(mg/L)

Volumetric productivity

(mg/L/h)

Yield on carbon

(mmol/mol sucrose)

BS168NU-Sd

–

166.88

6.95

0.80

BS168NU-S

–

372.30

15.51

1.75

racE

racE

405.83

16.91

1.93

murC

murC

421.58

17.57

1.98

H3

racE, murC

478.69

19.95

2.26

yrpC

yrpC

541.57

22.57

2.55

H2

yrpC, murC

566.41

23.60

2.69

H1

yrpC, racE

751.90

31.33

3.54