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Table 1 Strains used in this study

From: Enhancing surfactin production by using systematic CRISPRi repression to screen amino acid biosynthesis genes in Bacillus subtilis

Name

Genotype

Source/references

E. coli Trans T1

F-φ80(lacZ)ΔM15ΔlacX74hsdR(r −k , m +k )ΔrecA1398endA1tonA

TransGen Biotech

B. subtilis 168

trpC2

Laboratory stock

BS168NU

trpC2, ΔaraR::Para-neo, Δupp

Laboratory stock

MK3-MEP2-m

BS168NU, ΔyxlA::PlapS-menA, ΔydeO::P43-dxr-DN*-cat-araR

Laboratory stock

BS168NU-S

BS168NU, ΔydeO::P43-sfp

This work

BS168NU-Sd

BS168NU-S derivate, ΔlacA::Pxyl-dCaS9

This work

mmgA

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAmmgA

This work

yhfS

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAyhfS

This work

asnB

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAasnB

This work

asnH

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAasnH

This work

asnO

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAasnO

This work

nadB

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAnadB

This work

yhdR

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAyhdR

This work

racX

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAracX

This work

pyrB

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNApyrB

This work

pyrC

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNApyrC

This work

purF

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNApurF

This work

glnA

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAGlnA

This work

yrpC

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAyrpC

This work

racE

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAracE

This work

glmS

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAglmS

This work

ybcM

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAybcM

This work

murD

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAmurD

This work

murC

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAmurC

This work

bkdAA

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAbkdAA

This work

bkdAB

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAbkdAB

This work

H1

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAyrpC, Pveg-sgRNAracE

This work

H2

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAyrpC, Pveg-sgRNAmurC

This work

H3

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAracE, Pveg-sgRNAmurC

This work

H4

BS168NU-Sd derivate, ΔamyE::Pveg-sgRNAyrpC, Pveg-sgRNAracE, Pveg-sgRNAmurC

This work