Fig. 5
Effect of multiple gene inhibition on surfactin production in B. subtilis. a Schematic diagram of the construction for multiple gene inhibition by CRISPRi. b Surfactin production and OD600 of the recombinant strains in which the yrpC, racE and murC genes were inhibited in combination. The histogram represents the production of surfactin, and the scatter represents OD600. c The surfactin production per OD600 of the strains in which yrpC, racE and murC were inhibited in combination. The surfactin production and the cell growth of the recombinant strains were all determined 24 h after inoculation in 5 mL of semi-defined fermentation medium. “-” indicates the inhibition of a specific gene by CRISPRi