Skip to main content

Table 2 Strains used in this study

From: Efficient production of glycyrrhetinic acid in metabolically engineered Saccharomyces cerevisiae via an integrated strategy

Name Description Source
Cenpk2-1D MATα ura3-52 trp1-289 leu2-3,112 his3Δ1 MAL2-8C SUC2 EUROSCARF
Y0 Ppgk1-β-AS-Tadh1, Ptdh3-CYP88D6-Tcyc1, Padh1-CYP72A154-Tadh1 and Ptdh3-AtCPR1-Tcyc1 cassettes and LEU2 marker gene were integrated into rDNA site of Cenpk2-1D This study
This study
Y1 Ppgk1-β-AS-Tadh1, Ptdh3-OPCYP88D6-Tcyc1, Padh1-OPCYP72A154-Tadh1 and Ptdh3-AtCPR1-Tcyc1 cassettes and LEU2 marker gene were integrated into rDNA site of Cenpk2-1D This study
Y2 Ptdh3-E20+9-Tcyc1(Ptdh3-E20+9-Tcyc1), Ptef1-ERG1-Tpgk1 and Ppgk1-tHMG1-Tadh1 cassettes and URA3 marker gene were integrated into delta site of Y1 This study
Y4 Ptdh3-ERG8-Ttdh3, Padh1-E13-Tadh1 and Padh1-E10-Tadh1 cassettes and HIS3 marker gene were integrated into TRP1 site of Y1 This study
Y5 Padh1-ERG12-Tadh1, Ptdh3-Cyb5-Ttdh3, Ptef2- ERG19-Tcyc1 and Ppgk1-IDI- Tpgk1 cassettes and HIS3 marker gene were integrated into BTS1 site of Y1 This study
Y6 Ptdh3-E20+9-Tcyc1(Ptdh3-E20+9-Tcyc1), Ptef1-ERG1-Tpgk1 and Ppgk1-tHMG1-Tadh1 cassettes and URA3 marker gene were integrated into delta site of Y4 This study
Y7 Padh1-ERG12-Tadh1, Ptdh3-Cyb5-Ttdh3, Ptef2- ERG19-Tcyc1 and Ppgk1-IDI- Tpgk1 cassettes and HIS3 marker gene were integrated into BTS1 site of Y6 This study