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Table 1 Plasmids used in this study

From: Efficient production of glycyrrhetinic acid in metabolically engineered Saccharomyces cerevisiae via an integrated strategy

Name

Description

Source

pUC19L

Cloning vector with multiple cloning sites, Amp

Invitrogen

pATP423

2MICRON, HIS3

Ishii et al. [38]

pATP424

2MICRON, TRP1

Ishii et al. [38]

pATP425

2MICRON, LEU2

Ishii et al. [38]

pATP406

w/o yeast origin, URA3

Ishii et al. [38]

PRS423

2MICRON, HIS3

Christianson [39]

pUC19L-Ptef1-Tpgk1

Cloning Ptef1-Tpgk1 cassette into pUC19L

This study

pUC19L-Ptdh3-Tcyc1

Cloning Ptdh3-Tcyc1 cassette into pUC19L

This study

pUC19L-Ppgk1-Tadh1

Cloning Ppgk1-Tadh1 cassette into pUC19L

This study

pUC19L-Ptef2-Tcyc1

Cloning Ptef2-Tcyc1 cassette into pUC19L

This study

pUC19L-Ptdh3-Ttdh3

Cloning Ptdh3-Ttdh3 cassette into pUC19L

This study

pUC19L-Ptef2-ERG19-Tcyc1

Cloning Ptef2-ERG19-Tcyc1 cassette into pUC19L

This study

pUC19L-Ptdh3-ERG9+20-Tcyc1

Cloning Ptdh3-ERG9+20-Tcyc1cassette into pUC19L

This study

pUC19L-Ptdh3-ERG20+9-Tcyc1

Cloning Ptdh3-ERG20+9-Tcyc1 cassette into pUC19L

This study

pUC19L-Ptef1-ERG1-Tpgk1

Cloning Ptef1-ERG1-Tpgk1 cassette into pUC19L

This study

pUC19L-Ppgk1-tHMG1-Tadh1

Cloning Ppgk1-tHMG1-Tadh1 cassette into pUC19L

This study

pUC19L-Ppgk1-β-AS-Tadh1

Cloning Ppgk1-β-AS-Tadh1 cassette into pUC19L

This study

pUC19L-Ptdh3-CYP88D6-Tcyc1

Cloning Ptdh3-CYP88D6-Tcyc1 cassette into pUC19L

This study

pUC19L-Ptdh3-AtCPR1-Tcyc1

Cloning Ptdh3-CPR1-Tcyc1 cassette into pUC19L

This study

pATP406-ERG8

pATP406, expressing ERG8 gene by PTDH3

This study

pATP406-ERG10

pATP406, expressing ERG10 gene by PADH1

This study

pATP406-ERG12

pATP406, expressing ERG12 gene by PADH1

This study

pATP406-ERG13

pATP406, expressing ERG13 gene by PADH1

This study

pATP406-IDI1

pATP406, expressing IDI1 gene by PPGK1

This study

pATP406-CYB5

pATP406, expressing CY B5 gene by PTDH3

This study

pATP24- CYP72A154

PATP424, expressing CYP72A154 gene by PADH1

This study

pATP424- AtCPR1

pATP424, expressing AtCPR1 gene by PTDH3

This study

pATP424-β-AS

pATP424, expressing β-AS gene by PPGK1

This study

pATP424-OP-CYP72A154

pATP424, expressing OP-CYP72A154 gene by PADH1

This study

pATP424-OP88D6

pATP424, expressing OP-CYP88D6 gene by PTDH3

This study