Efficient chiral synthesis by Saccharomyces cerevisiae spore encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase in organic solvents

Table 1 Enantioselective synthesis of (R)-PE by yeast-spore coencapsulation of ADHs and free enzymes in aqueous medium

	Samples	Yield (%)^a	ee (%)^b	Reduction durations (h)^c	Productivity (mM h⁻¹ g⁻¹)
E228S SCRII	Free	93	> 99	8	77.9
	Encapsulated^d	89	> 99	6	99.4
	Encapsulated (10)	83	> 99	60	92.7
E228S-GDH^e	Free	95	> 99	6	106.1
	Co-encapsulated	93	> 99	2	311.6
	Co-encapsulated (10)	86	> 99	20	288.1

All reactions were carried out at 30 °C in 100 mM PBS buffer solution (pH 7.4) by using free ADHs (0.1 mg) and Spore_E228S or Spore_E228S-GDH (0.1 g). The concentration of AP was 8 g/L (≈ 67 mM)
^a% yield of (R)-PE was determined by HPLC
^bee: enantiomeric excess, the ee values of (R)-PE were determined using a chiral stationary phase on a Chiralcel OB-H column [3]
^cReaction duration was determined when the product (R)-PE was produced with the highest yield
^dThe value in parentheses denotes the times the encapsulated enzymes was recycled for reactions

ISSN: 1475-2859