Fig. 4From: Efficient chiral synthesis by Saccharomyces cerevisiae spore encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase in organic solventsAsymmetric synthesis of (R)-PE by SporeE228S-GDH in freezing and thawing cycles. The SporeE228S-GDH was frozen at − 20 °C and was thawed at room temperature. The freezing–thawing process was repeated at least once a day. Some of SporeE228S-GDH was used for enantiospecific reaction, and the rest was taken back at − 20 °CBack to article page