Table 3 Strains used in this work
From: Enhanced protopanaxadiol production from xylose by engineered Yarrowia lipolytica
Strains | Description | Source |
|---|---|---|
Y0 | Yarrowia lipolytica ATCC 201249: MATA, ura3-302, leu2-270, lys8-11, pex17-ha | [59] |
Y1 | Y0: Ku70 deletion::LUL | This study |
Y2 | Y1: zeta::exp1p-XYL1-xpr2t, gpd1p-XYL2-lip2t, tef1p-ylXKS-cyc1t | This study |
Y3 | Y1: zeta::exp1p-ylXYL1-xpr2t, gpd1p-ylXYL2-lip2t, tef1p-ylXKS-cyc1t | This study |
Y4 | An adaptation of xylose in Y2 | This study |
Y5 | An adaptation of xylose in Y3 | This study |
Y6 | The marker ura3 was recycled in Y4 | This study |
Y7 | Y6: rDNA::tef1p-DS-xpr2t, exp1p-PPDS-mig1t, gpd1p-ATR1-lip2t | This study |
Y8 | Y6: rDNA::tef1p-DS-xpr2t, exp1p-PPDS-linker-ATR1-lip2t | This study |
Y9 | The marker ura3 was recycled in Y8. | This study |
Y10 | Y9: POX1::Fbainp-tHMG1-xpr2t, gpd1p-erg9-cyc1t, exp1p-erg20-lip2t | This study |
Y11 | The marker ura3 was recycled in Y10. | This study |
Y12 | Y11: POX2::exp1p-TKL-mig1t, tef1p-TAL-lip2t | This study |
Y13 | The marker ura3 was recycled in Y12. | This study |
Y14 | Y13: POX3:: Pfba1-TX-cyc1t | This study |