Skip to main content

Table 2 Primers used in this study for the PCR check and mutagenesis

From: A genetically engineered Escherichia coli strain overexpressing the nitroreductase NfsB is capable of producing the herbicide D-DIBOA with 100% molar yield

Name

Description/Sequence (5´-3´)

Sources

Primers

Kt-ra

CGGCCACAGTCGATGAATCC

Datsenko and Wanner [22]

rcsA-f

GTGACCCATGTTGTTCCGTTTAG

This study

wzc-f

CGCCATATCGAACGCTTATG

This study

dgsA-f

CCGTCATCACTCAGGAGGTG

This study

glgA-f

CCGGAACTGGATATGTACGATC

This study

wzxE-f

GCAGAACGCGCATATGTTCTAC

This study

flgA-f

ATTGCGGACAGGTACAATTCAC

This study

flhA-f

TGTATCGACATGCGGAGATTG

This study

fliQ-f

GTGATAGCCAGCGTGTTGATG

This study

lrhA-f

TGCACGAGAGTGGAACAAGG

This study

ccmA-f

CTGACGATGGCACAGAATGAG

This study

citD-f

CCAGGAGATGCCGATATCCGC

This study

grxA-f

GCTTCCCTCTGCAAAGTGAGCC

This study

lapA-f

GCGAACGTTAACCATTGCTATC

This study

lapA-r

AACGGAACAGGTTTCCGAGCG

This study

kdsD-f

CGTGACTACAGCGTGATGTTG

This study

lpcA-f

AGCACCTGCCCGTACTTCTCGC

This study

arnA-f

CTGACCTTCGGACCACAATG

This study

rfbX-f

TGGTTCTGTCTGATATCGCTG

This study

wbbI-f

ACAGGTGGAGTCTCTATGTCG

This study

ybaY-f

GGCAGAAATGCGTGATGTGTGC

This study

cdsA-f

GATGTTCTCTGGCCCGATTTC

This study

clsA-f

TCCGTTCTACTCCGCTTCATG

This study

pgpA-f

TCACTGTGCCGGAACTGAACC

This study

fadR-f

GCAGGAGTGAGGCAAGTCTTG

This study

pfk I-f

TGGTTCAGGCACATATGGTG

This study

pfk II-f

CATAACGATGGCAGGAACTGTC

This study

sthA-f

CCCATCACGATGTCTGAATCC

This study

nuoA-f

TATCCTGGAGTCGTCAAGGATC

This study

nuoB-f

TCGCATGGTCAACCTCTATCC

This study

nuoE-f

CAGAGTCTGCGCATTCTTGAG

This study

nuoF-f

GCGTGGTCTGTCATATCAACG

This study

nuoG-f

GTGCAGTGGAGCCGTTACAG

This study

nuoN-f

GGCAGCTTCCAGGTTGTACC

This study

pgi-f

CCTGTAGCCGATGATGAACG

This study

narG-f

CCACGCTGTTTCAGAGCGTTAC

This study

H1P4-fliQ

TGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGATTCCGGGGATCCGTCGACC

This study

H2P1-fliQ

GTTCGCTTGTCACCTGCAACATAGTACGGCTACCCGATGATATACGGCAGTGTAGGCTGGAGCTGCTTCG

This study

Plasmids

pBAD-NfsB

pBAD vector with AmpR harbouring the nitroreductase NfsB

Valle [18]

pCP20

AmpR and CmR plasmid; thermal induction of FLP synthesis

Datsenko and Wanner [22]

pKD46

AmpR plasmid contains red system for homologous recombination

Datsenko and Wanner [22]

pKD13

KanR plasmid; was used for generating the cassette with kanamycine resistance gene

Datsenko and Wanner [22]

Constructed strain

lapAfliQ::kan

KanR mutant

This study

  1. ApR , KanRand CmR indicate ampicillin, kanamycin and chloramphenicol resistance
  2. aAll mutant strains were checked by the kt-r reverse primer of internal kanamycin resistance cassette [22]