Fig. 2
From: In vivo continuous evolution of metabolic pathways for chemical production
In vivo genotype diversification. a Modified natural mutagenesis system. Random mutagenesis with higher mutation rate is induced with mutators. b Plasmid-targeted mutagenesis system. DNA plasmid-DNAP pair is designed such that the ep-DNAP mutates only the targeted plasmid. c Genome-targeted mutagenesis system. A gene editor is bind to a targeting protein, usually a Cas protein to mutate only a specific locus in genome. d Recombinase targeted mutagenesis system. Native system in microbial cells is used to recombine or re-integrate mutated gene into the plasmid