Enhancement of cellulase production in Trichoderma reesei RUT-C30 by comparative genomic screening

Table 3 Cellulase production and hydrolysis of pretreated corn stover by RUT-C30, SS-II, Δ108642, and Δ56839

Strain	FPase activity (U/mL)	Secreted protein (g/L)	Glucose (g/L)^a	Glucose yield (%)^a	t-test^b
RUT-C30	1.66 ± 0.08	0.97 ± 0.05	26.9 ± 1.3	66.2 ± 3.2	–
SS-II	1.59 ± 0.10	1.27 ± 0.07	35.2 ± 1.2	86.6 ± 3.3	**
Δ108642	3.05 ± 0.18	1.53 ± 0.09	30.1 ± 1.3	74.1 ± 3.1	*
Δ56839	2.82 ± 0.16	1.62 ± 0.0	27.3 ± 1.1	67.1 ± 3.4	–

“—” indicates s no significant difference
^aHydrolysis of pretreated corn stover was performed with FPase loading (20 FPU/g dry biomass) for 48 h
^bSignificance analysis of glucose yield of T. reesei SS-II, Δ108642, and Δ56839 relative to T. reesei RUT-C30. Asterisks indicate significant differences (*p < 0.05, **p < 0.01, ***p < 0.001, Student’s t-test)

ISSN: 1475-2859