Table 2 List of FPase activities produced by T. reesei RUT-C30 mutants for genes affected by SNPs and indels in T. reesei SS-II

1

A → G (exon: synonymous variant)

Unknown protein

3.05 ± 0.16

+ 83.7%

***

2

T → A (promoter)

Zinc-dependent alcohol dehydrogenase

2.82 ± 0.14

+ 70.1

***

3

− 1: A (intron)

Alcohol dehydrogenase GroES

2.55 ± 0.13

+ 53.4

***

4

T → C (terminator)

NAD(P)-binding protein

2.45 ± .15

+ 47.3

**

5

− 16: GATGACGA TGATTTTC (terminator)

P-loop containing nucleoside triphosphate hydrolase protein

1.95 ± 0.078

+ 17.3

*

6

T → C (exon: Cys₁₉₃ → Arg)

Methyltransferase type 11

1.88 ± 0.11

+ 13.3

–

7

A → G (promoter)

Unknown protein

1.81 ± 0.11

+ 9.1

–

8

A → T (terminator)

Conidiospore surface protein

1.80 ± 0.14

+ 8.8

–

9

A → T (promoter)

Unknown protein

1.78 ± 0.11

+ 7.3

–

10

T → C (terminator)

Unknown protein

1.75 ± 0.15

+ 5.3

–

11

T → A (exon: Phe₁₄₇₅₉ → Leu)

AMP-dependent synthetase and ligase

1.75 ± 0.09

+ 5.2

–

12

− 1: A (terminator)

PLC-like phosphodiesterase

1.70 ± 0.13

+ 2.5

–

13

A → T (terminator)

Thioredoxin

1.70 ± 0.12

+ 2.3

–

14

− 12: ACAATGACATG (promoter)

Amylolytic gene expression activator

1.68 ± 0.12

+ 1.3

–

15

G → A (promoter)

Unknown protein

1.64 ± 0.098

− 1.2

–

16

G → A (promoter)

Unknown protein

1.62 ± 0.11

− 2.2

–

17

A → T (exon: Asp₂₂₇ → Val); A → T (exon: Gln₂₂₉ → Leu); G → T (exon: Gly₂₃₀ → Val)

A → T (exon: Gln₂₃₃ → Leu)

Unknown protein

1.62 ± 0.12

− 2.3

–

18

A → T (exon: Ile₁₂₀ → Asn)

Amino acid transporter

1.61 ± 0.086

− 2.8

–

19

− 1: C (intron); − 2: AG (terminator); − 1: T (terminator)

Pkinase-domain-containing protein

1.60 ± 0.095

− 3.5

–

20

T → G (promoter)

GDP dissociation inhibitor

1.59 ± 0.10

− 4.3

–

21

T → G (promoter)

Carbohydrate kinase

1.58 ± 0.095

− 4.6

–

22

C → G (terminator)

Unknown protein

1.58 ± 0.094

− 4.8

–

23

+ 1: T (promoter)

YVTN repeat-like/Quino protein amine dehydrogenase

1.58 ± 0.11

− 5.2

–

24

− 1: G (intron)

Unknown protein

1.56 ± 0.13

− 5.8

–

25

A → G (exon: Met₈₆₇ → Thr)

ABC transporter

1.54 ± 0.096

− 7.3

–

26

− 3: TAC (terminator)

Acyl-CoA N-acyltransferase

1.52 ± 0.075

− 8.3

–

27

− 6: GCAGCA (terminator)

Glycosyltransferase

1.51 ± 0.078

− 8.7

–

28

T → C (promoter)

Unknown protein

1.50 ± 0.088

− 9.2

–

29

T → G (promoter)

Unknown protein

1.48 ± 0.096

− 11.0

–

30

A → G (terminator)

Amidase signature enzyme

1.44 ± 0.098

− 13.1

–

31

G → C (promoter)

Root hair defective 3 GTP-binding protein

1.43 ± 0.094

− 13.8

–

32

− 1: A (intron)

Unknown protein

1.34 ± 0.067

− 19.5

**

33

C → T (promoter)

Class I S-adenosyl-l-methionine-dependent methyltransferase

1.28 ± 0.055

− 22.6

**

34

A → C (promoter)

Calnexin

1.20 ± 0.071

− 27.7

**

35

T → C (intron)

Adaptor protein complex AP-1 small subunit

1.17 ± 0.058

− 29.3

**

36

G → A (exon: Ser₁₄₀ → Asn)

Unknown protein

1.16 ± 0.065

− 30.1

**

37

A → G (exon: Phe₁₂₂ → Ser)

MFS general substrate transporter

1.15 ± 0.058

− 30.8

***

38

G → A (exon: Ser₇₃ → Leu)

Clathrin adaptor complex

1.15 ± 0.047

− 31.0

***

39

A → T (promoter)

Stress response element binding protein

0.94 ± 0.045

− 43.6

***

40

T → A (intron)

Translationally controlled tumor protein

0.92 ± 0.054

− 44.3

***

41

C → G (exon: His₄₃₈ → Asp)

Unknown protein

0.91 ± 0.038

− 45.4

***

42

G → A (intron)

Zinc finger, TFIIS-type

0.76 ± 0.040

− 54.5

***

43

T → A (terminator); T → G (terminator); T → G (terminator); − 1: T (terminator)

Apolipophorin-III and similar insect proteins

0.72 ± 0.038

− 56.5

***

44

− 1: T (promoter)

Alpha and gamma adaptin binding protein p34

0.68 ± 0.041

− 59.3

***

45

C → T (promoter)

Class II Histone H3 methyltransferase

0.58 ± 0.033

− 65.2

***

46

+ 1: C (terminator)

serine/threonine phosphatase

0.56 ± 0.025

− 66.3

***

47

C → T (promoter)

RAM signaling pathway protein

n.t.

48

A → G (promoter)

ubiquitin

n.t.

49

A → C (promoter)

unknown protein

n.t.

50

G → T (terminator)

RNA polymerase III transcription initiation factor complex

n.t.

51

+ 3: TCT (terminator)

rRNA helicase RRP3

n.t.

52

− 16: GATGACGATGATTTTC (terminator)

unknown protein

n.t.

53

T → G (exon: Ile₄₉ → Arg)

Calcium-responsive transcription coactivator

n.t.

54

+ 3: TCT (exon)

Transcriptional corepressor

n.t.

55

C → T (exon: synonymous variant)

WD40 repeat-like protein

n.t.

56

T → C (intron)

Ribosomal protein L39e

n.t.

57

A → T (intron)

RuvB-like helicase 2

n.t.