Esterification of geraniol as a strategy for increasing product titre and specificity in engineered Escherichia coli

Table 1 Description of E. coli plasmids and strains used in this study

Name	Description	References
Plasmids
pET28a::RhAAT	pColE1, Kan^R, T7-RhAAT	This study
pJBEI-6410	p15a, Amp^R, PlacUV5-EcAtoB-SaHMGS-SaHMGR-T1^a, Ptrc-ScMK-ScPMK-ScPMD-Ecidi-T1002^b, Ptrc-AgtrGPPS_-MsLS	Alonso-Gutierrez et al. [25]
pGER	p15a, Amp^R, PlacUV5-EcAtoB_-SaHMGS-SaHMGR-T1, Ptrc-ScMK-ScPMK_-ScPMD-Ecidi-T1002, Ptrc-AgtrGPPS_-ObGS	This study
Strains
BIOBlue	recA1, endA1 gyrA96 thi-1 hsdR17(r_k-, m_k +) supE44 relA1 lac [F’ proAB lacI^qZΔM15 Tn10(Tet^r)]	Bioline
C43 (DE3)	F-ompT hsdSB (rB- mB-) gal dcm (DE3)	Lucigen
DLG	C43 (DE3) harbouring pGER	This study
DLGA	C43 (DE3) harbouring pET28a::RhAAT	This study
DLGA1	C43 (DE3) harbouring pGER and pET28a::RhAAT	This study

In the plasmid description, subscript letters refer to the source of the gene as follows: Rh = Rosa hybrid; Ec = Escherichia coli; Sa = Staphylococcus aureus; Sc = Saccharomyces cerevisiae; Ab = Abies grandis; Ms = Mentha spicate; Ob = Ocimum basilicum
^aT1: both the rrnB T1 terminator and T7Te terminator
^bT002: terminator

ISSN: 1475-2859