Fig. 4
Monoterpene and monoterpene ester production in E. coli under fed-batch fermentation using a two-phase system. a Accumulation of geraniol (purple), nerol (blue), citronellol (red) and total monoterpene (grey) in E. coli strain DLG. b Accumulation of geranyl acetate (green) in E. coli strain DLGA1. Both cultures were induced at an OD600 of ~ 20 with either 50 µM IPTG (a) or 125 µM IPTG (b), followed by the addition of a 10% dodecane top layer. Culture (b) was further supplemented with 20 mM acetic acid. Product titres are based on the aqueous volume of the cultures