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Table 1 Plasmids used in this study

From: Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production

Name Key features Source
pZS2-123 cfp under control of PLtetO-1, yfp under control of PLlacO-1, rfp under control of Plac/ara-1, Kanr [20]
pibpfxsT7lucA ibpfxs::lucA reporter unit, Ampr [11]
pJB658 RK2 replicon, trfA gene, XylS/Pm regulator/promoter system, Ampr [25]
pVB-1E0B1 IL-1RA RK2 replicon, trfA gene with cop-271 mutation, XylS/Pm regulator/promoter system, hok-sok suicide system, Ampr Vectron Biosolutions
pAG001 RK2 replicon, trfA gene, hok-sok suicide system, Ampr This study
pAG002 pAG001 plasmid with rfp under control of XylS/Pm, Ampr This study
pAG014 pAG001 plasmid with cfp under control of ibpfxs, Ampr This study
pAG017 pAG001 plasmid with yfp under control of rpsJ, Ampr This study
pAG019 pAG001 plasmid with rfp under control of XylS/Pm and yfp under control of rpsJ, Ampr This study
pAG024 pAG001 plasmid with rfp under control of XylS/Pm and cfp under control of ibpfxs, Ampr This study
pAG028 pAG001 plasmid with cfp under control of ibpfxs and yfp under control of rpsJ, Ampr This study
pAG032 pAG001 plasmid with rfp under control of XylS/Pm, cfp under control of ibpfxs and yfp under control of rpsJ, Ampr This study