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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production

Fig. 6

Responsiveness of the PrpsJ and XylS/Pm reporter units during cultivation of P. putida KT2440 under induced conditions (a) and the course of the Pibpfxs activity during cultivation at 42 °C (b). Data represent relative fluorescence levels shown as a ratio of OD600nm normalized fluorescence values at different time points under conditions of induction or heat shock to the OD600nm normalized fluorescence intensity of the reference cultures (R) growing in the absence of the inducer at 30 °C. The OD600nm normalized fluorescence values were calculated based on measurements taken every 1 h up to 4 h after the induction and every 0.5 h up to 2 h after the temperature shift. The data presented are from three independent biological replica (average ± SD)

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