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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production

Fig. 3

Time-course-activity measurements of YFP expressed from Pibpfxs in E. coli BW25113 (pAG032) cultivated in the presence of different concentrations (0, 4, 8, 16, 32 and 64 μg/ml) of azetidine. Incorporation of azetidine during protein synthesis promotes formation of abnormal proteins that tend to misfold and aggregate. The OD600nm normalized fluorescence values were calculated based on the measurements taken every 1 h up to 4 h after addition of azetidine. The data presented are from three independent biological replicas (average ± SD)

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Microbial Cell Factories

ISSN: 1475-2859

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