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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production

Fig. 1

Genetic map of the pAG032 reporter plasmid (11,238 nt large) including representation of the control mechanisms of the three promoter/reporter pairs. The XylS/Pm regulator/promoter is inducible by benzoic acid derivatives such as m-toluic acid, while the PrpsJ and Pibpfxs promoters are responsive to intracellular concentrations of ppGpp and σ32, respectively. oriT, origin for conjugative transfer; bla, gene conferring ampicillin resistance; oriV, origin of vegetative replication; trfA; gene encoding an essential initiator protein TrfA; T, transcription terminators; hok/sok RNA, hok-sok suicide elements; Ps, σ70-dependent constitutive promoter; xylS, gene encoding the positive transcriptional regulator XylS

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