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Table 1 Strains used in this work

From: Boosting the biosynthesis of betulinic acid and related triterpenoids in Yarrowia lipolytica via multimodular metabolic engineering

Strains

Characteristics

Source

Y. lipolytica ATCC 201249

MATA, ura3-302, leu2-270, lys8-11, pex17-HA

Provided by professor Yuan Yingjin (Tianjin University, China)

YLJCC0

201249 Δku70::PTEFin-RcLUS-acot, hisG

This work

YLJCC1

YLJCC0 Δku80::PFBAin-BPLO-octt, hisG

This work

YLJCC2

YLJCC1 IntF::PTEFin-ATR2-lip1t

This work

YLJCC3

YLJCC1 IntF::PTEFin-CrCPR-lip1t

This work

YLJCC4

YLJCC1 IntF::PTEFin-GuCPR-lip1t

This work

YLJCC5

YLJCC1 IntF::PTEFin-LjCPR-lip1t

This work

YLJCC6

YLJCC1 IntF::PTEFin-MTR-lip1t

This work

YLJCC7

YLJCC0 Δku80::PFBAin-CrAO-octt, hisG

This work

YLJCC8

YLJCC7 IntF::PTEFin-ATR2-lip1t

This work

YLJCC9

YLJCC7 IntF::PTEFin-CrCPR-lip1t

This work

YLJCC10

YLJCC7 IntF::PTEFin-GuCPR-lip1t

This work

YLJCC11

YLJCC7 IntF::PTEFin-LjCPR-lip1t

This work

YLJCC12

YLJCC7 IntF::PTEFin-MTR-lip1t

This work

YLJCC13

YLJCC0 Δku80::PFBAin-MtAO12-octt, hisG

This work

YLJCC14

YLJCC13 IntF::PTEFin-ATR2-lip1t

This work

YLJCC15

YLJCC13 IntF::PTEFin-CrCPR-lip1t

This work

YLJCC16

YLJCC13 IntF::PTEFin-GuCPR-lip1t

This work

YLJCC17

YLJCC13 IntF::PTEFin-LjCPR-lip1t

This work

YLJCC18

YLJCC13 IntF::PTEFin-MTR-lip1t

This work

YLJCC19

YLJCC0 Δku80::PFBAin-VvAO15-octt, hisG

This work

YLJCC20

YLJCC19 IntF::PTEFin-ATR2-lip1t

This work

YLJCC21

YLJCC19 IntF::PTEFin-CrCPR-lip1t

This work

YLJCC22

YLJCC19 IntF::PTEFin-GuCPR-lip1t

This work

YLJCC23

YLJCC19 IntF::PTEFin-LjCPR-lip1t

This work

YLJCC24

YLJCC19 IntF::PTEFin-MTR-lip1t

This work

YLJCC25

YLJCC0 Δku80::PFBAin-VvAO17-octt, hisG

This work

YLJCC26

YLJCC25 IntF::PTEFin-ATR2-lip1t

This work

YLJCC27

YLJCC25 IntF::PTEFin-CrCPR-lip1t

This work

YLJCC28

YLJCC25 IntF::PTEFin-GuCPR-lip1t

This work

YLJCC29

YLJCC25 IntF::PTEFin-LjCPR-lip1t

This work

YLJCC30

YLJCC25 IntF::PTEFin-MTR-lip1t

This work

YLJCC31

YLJCC0 Δku80::PFBAin-BPLO-LjCPR-octt, hisG-URA3-hisG

This work

YLJCC32

YLJCC0 Δku80::PFBAin-BPLO-L1-LjCPR-octt, hisG-URA3-hisG

This work

YLJCC33

YLJCC0 Δku80::PFBAin-BPLO-L2-LjCPR-octt, hisG-URA3-hisG

This work

YLJCC34

YLJCC0 Δku80::PFBAin-BPLO-L3-LjCPR-octt, hisG-URA3-hisG

This work

YLJCC35

YLJCC0 Δku80::PFBAin-BPLO-L4-LjCPR-octt, hisG-URA3-hisG

This work

YLJCC36

YLJCC0 Δku80::PFBAin-LjCPR-BPLO-octt, hisG-URA3-hisG

This work

YLJCC37

YLJCC0 Δku80::PFBAin-LjCPR-L1-BPLO-octt, hisG-URA3-hisG

This work

YLJCC38

YLJCC0 Δku80::PFBAin-LjCPR-L2-BPLO-octt, hisG-URA3-hisG

This work

YLJCC39

YLJCC0 Δku80::PFBAin-LjCPR-L3-BPLO-octt, hisG-URA3-hisG

This work

YLJCC41

YLJCC0 Δku80::PFBAin-BPLO-MTR-octt, hisG-URA3-hisG

This work

YLJCC42

YLJCC0 Δku80::PFBAin-BPLO-L1-MTR-octt, hisG-URA3-hisG

This work

YLJCC43

YLJCC0 Δku80::PFBAin-BPLO-L2-MTR-octt, hisG-URA3-hisG

This work

YLJCC44

YLJCC0 Δku80::PFBAin-BPLO-L3-MTR-octt, hisG-URA3-hisG

This work

YLJCC45

YLJCC0 Δku80::PFBAin-BPLO-L4-MTR-octt, hisG-URA3-hisG

This work

YLJCC46

YLJCC0 Δku80::PFBAin-MTR-BPLO-octt, hisG-URA3-hisG

This work

YLJCC48

YLJCC0 Δku80::PFBAin-MTR-L2-BPLO-octt, hisG-URA3-hisG

This work

YLJCC49

YLJCC0 Δku80::PFBAin-MTR-L3-BPLO-octt, hisG-URA3-hisG

This work

YLJCC50

YLJCC0 Δku80::PFBAin-MTR-L4-BPLO-octt, hisG-URA3-hisG

This work

YLJCC51

YLJCC5 rDNA::PEXP-ERG1-xpr2t

This work

YLJCC52

YLJCC5 rDNA::PEXP-ERG1-xpr2t, PTEFin-ERG9-lip2t

This work

YLJCC53

YLJCC5 rDNA::PEXP-ERG1-xpr2t, PTEFin-ERG9-lip2t, PTEFin-HMG1-octt

This work

YLJCC54

YLJCC6 rDNA::PEXP-ERG1-xpr2t

This work

YLJCC55

YLJCC6 rDNA::PEXP-ERG1-xpr2t, PTEFin-ERG9-lip2t

This work

YLJCC56

YLJCC6 rDNA::PEXP-ERG1-xpr2t, PTEFin-ERG9-lip2t, PTEFin-HMG1-octt

This work

YLJCC63

YLJCC5 IntE::PTEFin-EMC-pex20t, hisG-URA3-hisG

This work

YLJCC64

YLJCC5 IntE::PTEFin-EMT-pex20t, hisG-URA3-hisG

This work

YLJCC65

YLJCC5 IntE::PTEFin-Rtme-pex20t, hisG-URA3-hisG

This work

YLJCC66

YLJCC5 IntE::PTEFin-Gapc-pex20t, hisG-URA3-hisG

This work

YLJCC67

YLJCC6 IntE::PTEFin-EMC-pex20t, hisG-URA3-hisG

This work

YLJCC68

YLJCC6 IntE::PTEFin-EMT-pex20t, hisG-URA3-hisG

This work

YLJCC69

YLJCC6 IntE::PTEFin-Rtme-pex20t, hisG-URA3-hisG

This work

YLJCC70

YLJCC6 IntE::PTEFin-Gapc-pex20t, hisG-URA3-hisG

This work

YLJCC71

YLJCC5 IntE::PTEFin-ACL1-pex20t, hisG-URA3-hisG

This work

YLJCC72

YLJCC5 IntE::PTEFin-ACL2-pex20t, hisG-URA3-hisG

This work

YLJCC73

YLJCC5 IntE::PTEFin-PXA1-pex20t, hisG-URA3-hisG

This work

YLJCC74

YLJCC5 IntE::PTEFin-MFE1-pex20t, hisG-URA3-hisG

This work

YLJCC75

YLJCC5 IntE::PTEFin-PEX10-pex20t, hisG-URA3-hisG

This work

YLJCC76

YLJCC5 IntE::PTEFin-POT1-pex20t, hisG-URA3-hisG

This work

YLJCC77

YLJCC5 IntE::PTEFin-TGL3-pex20t, hisG-URA3-hisG

This work

YLJCC78

YLJCC6 IntE::PTEFin-ACL1-pex20t, hisG-URA3-hisG

This work

YLJCC79

YLJCC6 IntE::PTEFin-ACL2-pex20t, hisG-URA3-hisG

This work

YLJCC80

YLJCC6 IntE::PTEFin-PXA1-pex20t, hisG-URA3-hisG

This work

YLJCC81

YLJCC6 IntE::PTEFin-MFE1-pex20t, hisG-URA3-hisG

This work

YLJCC82

YLJCC6 IntE::PTEFin-PEX10-pex20t, hisG-URA3-hisG

This work

YLJCC83

YLJCC6 IntE::PTEFin-POT1-pex20t, hisG-URA3-hisG

This work

YLJCC84

YLJCC6 IntE::PTEFin-TGL3-pex20t, hisG-URA3-hisG

This work

YLJCC85

YLJCC53 IntE::PTEFin-EMT-pex20t, hisG-URA3-hisG

This work

YLJCC86

YLJCC53 IntE::PTEFin-Rtme-pex20t, hisG-URA3-hisG

This work

YLJCC87

YLJCC56 IntE::PTEFin-EMT-pex20t, hisG-URA3-hisG

This work

YLJCC88

YLJCC56 IntE::PTEFin-Rtme-pex20t, hisG-URA3-hisG

This work

YLJCC89

YLJCC53 IntE::PTEFin-ACL2-pex20t, hisG-URA3-hisG

This work

YLJCC90

YLJCC53 IntE::PTEFin-PXA1-pex20t, hisG-URA3-hisG

This work

YLJCC91

YLJCC53 IntE::PTEFin-MFE1-pex20t, hisG-URA3-hisG

This work

YLJCC92

YLJCC53 IntE::PTEFin-POT1-pex20t, hisG-URA3-hisG

This work

YLJCC93

YLJCC53 IntE::PTEFin-TGL3-pex20t, hisG-URA3-hisG

This work

YLJCC94

YLJCC56 IntE::PTEFin-ACL2-pex20t, hisG-URA3-hisG

This work

YLJCC95

YLJCC56 IntE::PTEFin-PXA1-pex20t, hisG-URA3-hisG

This work

YLJCC96

YLJCC56 IntE::PTEFin-MFE1-pex20t, hisG-URA3-hisG

This work

YLJCC97

YLJCC56 IntE::PTEFin-POT1-pex20t, hisG-URA3-hisG

This work

YLJCC98

YLJCC56 IntE::PTEFin-TGL3-pex20t, hisG-URA3-hisG

This work

YLJCC99

YLJCC0 IntF::PTEFin-LjCPR-lip1t

This work

YLJCC100

YLJCC0 IntF::PTEFin-MTR-lip1t

This work

YLJCC101

YLJCC0 Δku80::PFBAin-BPLO-ER-octt, hisG; IntE::PTEFin-LjCPR-ER-pex20t, hisG-URA3-hisG

This work

YLJCC102

YLJCC0 Δku80::PFBAin-BPLO-ER-octt, hisG; IntE::PTEFin-MTR-ER-pex20t, hisG-URA3-hisG

This work

YLJCC103

201249 Δku80::PFBAin-BPLO-sfGFP-octt, hisG-URA3-hisG

This work

YLJCC104

201249 Δku80::PFBAin-LjCPR-sfGFP-octt, hisG-URA3-hisG

This work

YLJCC105

201249 Δku80::PFBAin-MTR-sfGFP-octt, hisG-URA3-hisG

This work