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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production

Fig. 6

Influence of the maximum oxygen transfer capacity on 2,3-butanediol production with Bacillus licheniformis DSM 8785. Calculated maximum total 2,3-butanediol (a), glycerol (b) and acetoin concentrations (c), the sum of accumulated by-products (d), the time until glucose was depleted (e) and the space–time yield (f) are shown in dependency of the maximum oxygen transfer capacity during the 2,3-butanediol production phase. Each data point is derived from one of 28 individual experiments and refers to the time point of glucose depletion. 2,3-Butanediol is shown as sum of the stereoisomers. The time of glucose depletion is determined from the sudden drop of the respiratory quotient, which was measured in biological duplicates. Acetoin, 2,3-butanediol and glycerol concentrations at this time were calculated from offline samples as illustrated in Fig. 4. The space–time yield was calculated from the time of glucose depletion and the maximum 2,3-butanediol concentration. The solid lines represent the fitted linear (a, b), exponential (c, e) or cubic (f) correlations. The solid line in e was calculated as sum of the fitted lines from b and c. Error margins (10% for a, d and e; 3 g/L for bd) are represented by dashed lines. To account for the increase of metabolite concentrations due to evaporation of water, all concentrations were corrected accordingly and referred to the initial filling volume

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