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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production

Fig. 4

Glucose consumption and product formation during the cultivation of Bacillus licheniformis DSM 8785. As indicated by the left vertical dotted line, the shaking frequency was reduced from 350 to 100 rpm after 3 h. Data on oxygen transfer rate (OTR) and respiratory quotient (RQ) (a), glucose and total 2,3-butanediol concentration (b) and glycerol and acetoin concentration (c) are depicted. The solid lines represent linear correlations during the 2,3-butanediol production phase (between the dotted vertical lines) with R2 ≥ 0.99 for glucose, 2,3-butanediol and glycerol and R2 = 0.89 for acetoin. Filling of symbols represents data points that were (closed symbols) or were not (open symbols) considered for linear correlation. 2,3-Butanediol is shown as sum of the stereoisomers. The RQ is only shown for OTR > 1 mmol/L/h. To account for the increase of metabolite concentrations due to evaporation of water, all concentrations were corrected accordingly and referred to the initial filling volume. Cultivation conditions: 250 mL unbaffled shake flasks, temperature: 37 °C, shaking frequency: 350/100 rpm, shaking diameter: 50 mm, filling volume: 30 mL, Nakashimada medium with 100 mM MES buffer

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