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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Developing a single strain for in vitro salvage synthesis of NAD+ at high temperatures and its potential for bioconversion

Fig. 7

NAD+/NADH dependent redox reaction without exogenous NAD+. a Scheme of the NAD+/NADH-dependent redox reaction with the NAD+ salvage cocktail is shown. GDH and LDH represent glucose dehydrogenase and lactate dehydrogenase, respectively. Chemical substances are shown in the box (GLC, glucose; GDL, glucono δ-lactone; PYR, pyruvate; LAC, lactate). Lactate (b) and glucose (c) concentrations were measured every 8 h for 40 h with the enzyme cocktail (+ cocktail) or in the negative control (− cocktail). Error bars represent standard errors calculated from triplicate measurements

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