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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Developing a single strain for in vitro salvage synthesis of NAD+ at high temperatures and its potential for bioconversion

Fig. 5

PPK screening and their polyphosphate dependency. a Spectrometric quantification of PPK activity is illustrated. G6P; glucose-6-phosphate, 6-PG; 6-phosphogluconic acid. b The activity of PPK from T. thermophilus and R. marinus are shown. Following polyphosphate were used in the assay; PolyP-1 (purchased from Nacalai Tesque, Cat: 27704-15; Japan); PolyP-2 (FUJI Film, Cat: 194-05935; Japan); PolyP-3 (Sigma, Cat: 28-2880-5; Germany); and PolyP-4 (Sigma, Cat: 1.06529; Germany). PolyP-5 was prepared as describe elsewhere [18]

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Microbial Cell Factories

ISSN: 1475-2859

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