Fig. 4From: Developing a single strain for in vitro salvage synthesis of NAD+ at high temperatures and its potential for bioconversionOverall performance of the enzyme cocktail and its thermal stabilities. The activity of the enzyme cocktail to synthesize NAD+ from NAM and ADP-ribose is shown. The activity of the salvage cocktail was measured after incubation at 60 °C for 0, 24 and 48 h. Residual activities after 24 and 48 h were normalized to the activity without incubation (0 h). The absolute activity was calculated against wet cell weight used for the preparation of enzyme cocktail. One unit is defined as the activity to produce 1 µmol of NAD+ per minute. The error bar represents standard error calculated from triplicate measurementsBack to article page